| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shipe, Dylayn J (13)
|
AMRGSY |
5:03.09 |
5:01.89 |
-1.20 |
| 2 |
Shipe, Bryony S (13)
|
AMRGSY |
5:12.91 |
5:07.53 |
-5.38 |
| 3 |
Bacu, Claire P (13)
|
AMANSC |
5:45.55 |
5:24.06 |
-21.49 |
| 4 |
Barton, Isabelle R (14)
|
AMRGSY |
5:17.82 |
5:28.13 |
10.31 |
| 5 |
Dassanaike, Mikaela T (13)
|
AMANSC |
6:18.78 |
5:28.79 |
-49.99 |
| 6 |
Alexander, Gabrielle C (14)
|
AMSVSC |
6:30.55 |
5:28.82 |
-61.73 |
| 7 |
Dunn, Sydney A (13)
|
AMHD |
6:31.08 |
5:38.51 |
-52.57 |
| 8 |
Szymanski, Tessa A (13)
|
AMBYAC |
5:53.56 |
5:43.92 |
-9.64 |
| 9 |
Bruce, Madison S (13)
|
AMHD |
6:03.82 |
6:06.08 |
2.26 |
| 10 |
Vitale, Sarah B (13)
|
AMSASC |
6:48.87 |
6:21.13 |
-27.74 |
| 11 |
Roth, Ella S (14)
|
AMRXA |
6:00.99 |
6:31.53 |
30.54 |